Epson C82302/04 (Serial I/F) Manual do Utilizador Página 53

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restriction sites. Dideoxy sequencing can also be performed on
the amplified product.
Instrument Description
The GeneAmp PCR System 9600 contains a programmable
heating and cooling block, which
rapidly and uniformly
heats
and cools PCR samples.
A Look at the Sample Block
The sample block is a 96-well aluminum block. Up to 96 samples
in MicroAmp Reaction Tubes (0.2 mL) are loaded in a sample
tray and placed in the block. When a tray is in place, the heated
cover is moved forward over the sample block area and exerts an
even pressure on all tubes.
Figure 1-2
Sample block and heated cover.
There are 17 holes within the block for coolant flow: eight of the
holes are used for rapid sample block cooling (ramp cooling) and
the remaining nine are for cold-biasing the system. A Kapton
heater beneath the sample block is responsible for rapid heating
and control of the sample block. The heater is designed so that
Heated Cover
Sample Block
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