Epson C82302/04 (Serial I/F) Manual do Utilizador descarregar pdf (Página 119)

4-13

Figure 4-7

An AUTO program used to increase hold time.

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PCR System 9600 1
Part No 2
Version Date 2
Back of Instrument 7
LEFT SIDE 9
BACK VIEW 10
United States (FCC) 11
Operating Precautions 12
Technical Support 16
To Contact 17
Outside North America 18
To Reach 20
Through the 20
Internet 20
To Obtain 21
Documents on 21
Users Manual Summary 23
Sample Block 26
Sample Tubes 26
Comparing Files and Programs 27
Function Keys 28
File Storage 29
Operation 29
Figure 1 31
System 9600 31
If your change in 32
Subtract this time from your 32
Thermal Cycler hold time: 32
Contents 1 35
Illustrations 11 43
Preface: Using the Manual P 47
GeneAmp PCR System 9600: 48
Conventions 49
Notes and Warnings 50
Introducing the GeneAmp 51
PCR System 9600 1 51
Principles of Operation 52
Instrument Description 53
A Look at the Control Panel 54
Installing the GeneAmp 57
PCR System 9600 2 57
Unpacking 58
Parts Supplied 58
within the U.S 59
Power Requirements 60
Proper Location 61
Setting Up the Printer 62
Figure 2-1 64
Figure 2-2 65
User's manual 67
Time HH:MM 69
Date MM/DD/YY 69
Runtime printer OFF 69
Runtime beeper ON 69
Heater and Chiller Tests 75
Tutorial: Customizing a 79
PCR CYCL Program 3 79
Before You Start 80
What is a CYCL Program? 81
Figure 3-2 82
Starting Up the Instrument 83
Getting to the CYCL Program 84
A Look at the CYCL Program 87
Setpt #2 Ramp 0:00 88
55.0C Hold 0:30 88
Setpt #3 Ramp 0:00 88
72.0C Hold 0:30 88
To d i s p lay 89
Figure 3-4 91
Customizing the CYCL Program 92
To change NO to 96
YES and vice versa 96
Program Type 97
Storing Your CYCL Program 98
Assigned Program Number (1) 99
Starting a Trial Run 100
Figure 3-5 101
Ramp to 96.5C xx.xC 103
0:30 Cycle 1 103
0:22 Cycle 1 103
Total cycles in program 104
Current setpoint number 104
The Next Step 105
Software Basics 4 107
Reviewing Keypad Basics 108
A Close Look at the Main Menu 110
Figure 4-2 111
Storing Programs and Methods 113
The HOLD Program 114
Figure 4-4 115
Figure 4-5 115
CYCL: The Cycle Program 116
Figure 4-6 117
A common CYCL program 117
The AUTO Program 118
Figure 4-7 119
PCR Methods 120
Setting Up PCR Programs 121
Figure 5-1 123
PCR System 9600 123
Figure 5-2 125
Figure 5-3 127
Editing Keys 128
Creating and Editing Programs 129
Program Type: 130
Storing the Program 131
Enter program # x 131
Protect program? NO 131
Enter user # 132
Prog # x User #xxxx 132
OK to store? YES 132
Enter Program # x 134
Prog x is protected 135
Enter user # _ 135
Creating and Editing Methods 137
(Method) 140
Printing a Method 145
DATA option 146
Software Shortcuts 148
Returns you to the main menu 148
CYCL#??? xx.xC 149
UN-STORE-PRINT-HOME 149
Program Number (26) 150
Running PCR Samples6 167
Loading Samples 168
Figure 6-1 169
Figure 6-2 170
Figure 6-3 171
Figure 6-4 172
Figure 6-5 173
Turning the cover knob 173
Using the Cap-Installing Tool 174
Figure 6-6 175
Starting the Run 177
! WARNING ! 181
Figure 6-9 183
Figure 6-10 184
Using the MORE key 184
Figure 6-11 184
Figure 6-12 185
Pausing a Run 186
Stopping a Run 186
CYCL# 26 Abort xx.xC 187
History 188
Utilities 7 189
Getting to the Utilities Menu 190
Figure 7-1 191
The Utilities functions 191
The Directory Function (DIR) 192
Directory 194
Enter program # 1 194
HOLD #14 196
HOLD #26 196
Figure 7-3 197
CYCL #6 User#7777 199
HOLD #5 User#7777 199
Figure 7-4 200
Directory function 203
Figure 7-6 205
The Configuration function 205
Figure 7-7 214
Start clock within 215
C of setpoint 215
Rxn vol=100uL T= 9.5 217
Rxn vol= 50uL T= 7.0 217
Rxn vol= 20uL T= 5.0 217
Enter test # 224
Figure 7-8 225
Figure 7-9 229
Figure 7-10 232
Figure 7-11 234
CYCL program 234
Figure 7-12 235
History Print 237
Heater Test Blk=xx.x 239
Heater Test Passed 239
Chiller Test Blk=xx.x 241
Chiller Test Passed 241
System Performance 243
IEW-RUN-PRINT 243
The Delete Function (DEL) 245
Delete 246
Enter program #xxx 246
Can't delete file xxx 248
Linked in method xxx! 248
User #xxxx 248
Delete all progs?YES 248
Maintenance 8 251
Cleaning the Sample Wells 252
Cleaning the Heated Cover 253
Equipment Required 254
Figure 8-1 255
Select Option 9600 256
UN-CREATE-EDIT-UTIL 256
Record Temperature 259
Time=MM:SS Blk=95.0C 259
Test Results 260
Temperature Uniformity Test 263
Figure 8-5 264
Figure 8-7 266
Test Resul ts 267
Changing External Fuses 268
Fuse Holders 268
Troubleshooting 9 269
(continued) 271
If the Instrument Turns Off 273
Specifications 10 275
Power Requirements 276
Control Panel 276
Sample Temperature Range 277
Sample Temperature Accuracy 277
Displayed Sample Temperature 277
Sample Temperature Overshoot 277
Ramp Time Repeatability 278
Printer Interface 279
Temperature Calibration 279
Index-10 290
Index-11 291
Index-12 292
business 294

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